digital imaging for scanning electron microscopy  (CRC Press Inc)

Journal:

Article Title: Nanomechanical properties and mineral concentration in articular calcified cartilage and subchondral bone

doi: 10.1046/j.1469-7580.2003.00193.x

Figure Lengend Snippet: qBSE (20 kV) image of PM field, 67-year-old male. (a) Grey levels were scaled using the halogenated dimethacrylate standards and ranked into eight classes: the colour look-up table allows visual inspection of bone mineralization density from the qBSE–SEM images. (b) Histogram of this image field using the same colour look-up table. (c) Topographic BSE image of the same field to permit location of indents (here, in two adjacent arrays): five high load indents at end of each array simplify the search process. (d) Indent array distribution overlain on a qBSE image to extract BSE grey-level measurements at the (−220 pixels) indent sites (white patches: grey patches rejected). Labels: C = ACC, B = SCB, H = hyaline articular cartilage, M = marrow space in bone domain. Fieldwidths (a, c, d) = 594 μm.

Article Snippet: The ages of the individuals ranged from 55 to 89 years ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Post mortem Osteoarthritic Sex 4 male, 1 unknown 6 female Median age (years) 76 70 Age range (years) 67–85 55–89 Side 3 contralateral pairs, 1 unknown 2 RHS, 3 LHS, 1 unknown Open in a separate window RHS = right hand side; LHS = left hand side. caption a8 Summary of patient data Four-millimetre-thick slices cut in the frontal plane were photographed, radiographed, dehydrated in ethanol, embedded in PMMA, trimmed, diamond micromilled to an optically flat finish with the back parallel to the front of the block, and carbon coated for automated digital scanning electron microscopy (SEM) using backscattered electron (BSE) imaging (Zeiss DSM962 with Kontron IBAS external computer control, 20 kV, 0.5 nA, 17-mm working distance).

Techniques: